A shark antibody heavy chain encoded by a nonsomatically rearranged VDJ is preferentially expressed in early development and is convergent with mammalian IgG

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Rumfelt, L. L., Avila, D., Diaz, M., Bartl, S., McKinney, E. C., & Flajnik, M. F. (2001). A shark antibody heavy chain encoded by a nonsomatically rearranged VDJ is preferentially expressed in early development and is convergent with mammalian IgG. Proceedings of the National Academy of Sciences of the United States of America, 98(4), 1775-1780. doi:10.1073/pnas.98.4.1775
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TitleA shark antibody heavy chain encoded by a nonsomatically rearranged VDJ is preferentially expressed in early development and is convergent with mammalian IgG
AuthorsL. Rumfelt, D. Avila, M. Diaz, S. Bartl, E. McKinney, M. Flajnik
AbstractIn most vertebrate embryos and neonates studied to date unique antigen receptors (antibodies and T cell receptors) are expressed that possess a limited immune repertoire. We have isolated a subclass of IgM, IgM1gj, from the nurse shark Ginglymostoma cirratum that is preferentially expressed in neonates. The variable (V) region gene encoding the heavy (H) chain underwent V-D-J rearrangement in germ cells "germline-joined"). Such H chain V genes were discovered over 10 years ago in sharks but until now were not shown to be expressed at appreciable levels; we find expression of H1gj in primary and secondary lymphoid tissues early in life, but in adults only in primary lymphoid tissue, which is identified in this work as the epigonal organ. H1gj chain associates covalently with light (L) chains and is most similar in sequence to IgM H chains, but like mammalian IgG has three rather than the four IgM constant domains; deletion of the ancestral IgM C2 domain thus defines both IgG and IgM1gj. Because sharks are the members of the oldest vertebrate class known to possess antibodies, unique or specialized antibodies expressed early in ontogeny in sharks and other vertebrates were likely present at the inception of the adaptive immune system.
JournalProceedings of the National Academy of Sciences of the United States of America
Date2001
Start page1775
End page1780
ISSN0027-8424
Subjectsimmunoglobulin G, immunoglobulin heavy chain, immunoglobulin light chain, immunoglobulin M, lymphocyte antigen receptor, T lymphocyte receptor, animal cell, animal experiment, article, controlled study, embryo development, gene rearrangement, genetic code, genetic variability, germ cell, immune system, lymphoid tissue, nonhuman, nucleotide sequence, perinatal development, priority journal, promoter region, sequence analysis, sequence homology, shark, T lymphocyte, Amino Acid Sequence, Animals, Animals, Newborn, Exons, Gene Dosage, Gene Expression, Gene Rearrangement, B-Lymphocyte, Heavy Chain, Germ Cells, Immunoglobulin Heavy Chains, Immunoglobulin Joining Region, Immunoglobulin Variable Region, Mammals, Molecular Sequence Data, Multigene Family, Sharks, Animalia, Chondrichthyes, Ginglymostoma cirratum, Mammalia, unidentified agents, Vertebrata
NoteCited By (since 1996):41, CODEN: PNASA, Fish and Fisheries

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