Clonal diversity in introduced populations of an Asian sea anemone in North America

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Ting, J. H., & Geller, J. B. (2000). Clonal diversity in introduced populations of an Asian sea anemone in North America. Biological Invasions, 2(1), 23-32. doi:10.1023/A:1010085406539
Metadata
TitleClonal diversity in introduced populations of an Asian sea anemone in North America
AuthorsJ. Ting, J. Geller
AbstractPrevious reports hypothesized that introduced populations of the Asian sea anemone Diadumene lineata (Verill, 1870) which reproduces by fission, are often monoclonal or to be composed of few clones. To test this hypothesis, sea anemones were collected from thirteen sites in three non-native regions and one native region: Chesapeake Bay, New England, central California, and Japan. The internal transcribed spacer (ITS) region separating nuclear ribosomal RNA genes was amplified from each individual using PCR and surveyed for DNA sequence variation using single strand conformational polymorphism analysis (SSCP). Fifty-six distinct electrophoretic banding patterns were found in 268 anemones, and each pattern was considered a different genotype. The number of genotypes in a population ranged from one to thirteen. Only one sample (York River, Chesapeake Bay, n = 10) was monoclonal, although six populations were dominated (>50%) by single genotype. Only four genotypes were found in more than one population, and these were confined to single regions. Walker Creek, California was sampled in 1995 and 1997 and no genotypes were found in both years, suggesting rapid shifts in genotype frequency. We conclude that multiple genotypes of D. lineata have invaded North America and that the primary importance of clonal growth for introduced populations is the production of colonizing propagules.
JournalBiological Invasions
Date2000
Volume2
Issue1
Start page23
End page32
ISSN1387-3547
SubjectsAsia, clonal variation, genotype, geographic distribution, internal transcribed spacer, North America, plant invasion, polymerase chain reaction, ribosomal RNA, single strand conformation polymorphism, United States
NoteCited By (since 1996):8, Invertebrates, CODEN: BLINF

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